Previous Training Courses

Training course for cryopreservation of mouse sperm and embryos

We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including highly efficient superovulation, in vitro fertilization, cryopreservation of mouse sperm and embryos. We will also demonstrate micro-insemination operation and nuclear-transplantation cloning.

Training course on basic technologies required for Arabidopsis research

This is a training course for the researchers who are willing to study the plant science using Arabidopsis.

“Training course for human iPS cells” FY2023

Human iPS cells cannot be cultured with ordinary culture method and it requires special skills. This course provides a lecture and practical training for the method of passaging human iPS cells with feeder cells.

Some of human iPS cells (including iPS cells derived from patients) are cryopreserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of conventional slow-cooling method which is most commonly used in cell culture. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to cryopreserve human iPS cells with a vitrification method and how to thaw them in 1-day course.

Training course for rapid identification of yeasts/filamentous fungi by MALDI-TOF MS

Rapid identification of microbes by MALDI-TOF MS has been recently popular as it provides a fast and cost-effective alternative solution for nucleotide sequencing. This training course includes lecture and practice for researchers/technicians who are to perform rapid identification of yeasts and filamentous fungi by MALDI-TOF MS.

Technical training on phylogenetic analysis of fungi

This course presents technical methods for phylogenetic analysis of fungi.

The Modified SHIRPA Technical Training Course

The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc.

Training course for cryopreservation of mouse sperm and embryos

We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including highly efficient superovulation, in vitro fertilization, cryopreservation of mouse sperm and embryos. We will also demonstrate micro-insemination operation and nuclear-transplantation cloning.

The Modified SHIRPA Technical Training Course

The Modified SHIRPA is one of the simple and quick methods for evaluating comprehensive mouse phenotypic traits on mouse morphology, behavior, sensory responses, etc.

Technical training course for ICSI (intracytoplasmic sperm injection) of mice

Technical training course for mouse ICSI (intracytoplasmic sperm injection) by Piezo-assisted micromanipulation.

Technical training course for the grafting technique for Arabidopsis plants

This course presents basic methods for Arabidopsis grafting.

Training course for genetically-modified mouse generation using genome editing technology
– TAKE method for mouse zygote electroporation –

We will provide hands-on training of basic laboratory techniques to produce genetically-modified mouse strains, including mouse zygote thawing, electroporation, and embryo transfer. We will also give a brief lecture on the basics of genome editing.

“Training course for human iPS cells” FY2019

Human iPS cells cannot be cultured with ordinary culture method and it requires special skills. This course provides a lecture and practical training for the method of passaging human iPS cells with feeder cells.

Some of human iPS cells (including iPS cells derived from patients) are cryopreserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of conventional slow-cooling method which is most commonly used in cell culture. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to cryopreserve human iPS cells with a vitrification method and how to thaw them in 1-day course.

Training course for basic technologies required for Arabidopsis research
Optional course: Training course for cultivation of Brachypodium distachyon Bd21

This is a training course for the researchers who are willing to study the plant science using Arabidopsis.
<Optional course (at your request)>
Trainees will learn the cultivation procedure for Brachypodium distachyon, a model of monocot plants.

Technical training course for maintenance of plant cell cultures and transformation of Arabidopsis T87 cells
Optional course: Technical training course for the cryopreservation of tobacco BY-2 cells

This course presents basic methods to maintain Arabidopsis T87 and Tobacco BY2 suspension cultured cells (day 1) and applied methods for transformation technology of T87 cell (day 2).
<Optional course (at your request)>
Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.