Technical Training Courses

Previous Training Courses

Technical training course for ICSI (intracytoplasmic sperm injection) of mice

Technical training course for mouse ICSI (intracytoplasmic sperm injection) by Piezo-assisted micromanipulation.

Host Division: Bioresource Engineering Division

Dates Number of trainees
From November 25 to 27, 2019  (3 consecutive days) 4 people
Technical training course for the grafting technique for Arabidopsis plants

This course presents basic methods for Arabidopsis grafting.

Host Division: Experimental Plant Division

Dates Number of trainees
From November 13, 2019  (One day) 6 people
Training course for genetically-modified mouse generation using genome editing technology
– TAKE method for mouse zygote electroporation –

We will provide hands-on training of basic laboratory techniques to produce genetically-modified mouse strains, including mouse zygote thawing, electroporation, and embryo transfer. We will also give a brief lecture on the basics of genome editing.

Host Division: Experimental Animal Division

Dates Number of trainees
From September 9, 2019  (One day) 4 people
“Training course for human iPS cells” FY2019

Human iPS cells cannot be cultured with ordinary culture method and it requires special skills. This course provides a lecture and practical training for the method of passaging human iPS cells with feeder cells.

Some of human iPS cells (including iPS cells derived from patients) are cryopreserved with a vitrification method in our repository. The procedures of the vitrification method are specific and quite different from the procedures of conventional slow-cooling method which is most commonly used in cell culture. Consequently, the thawing procedures for the cells preserved with the vitrification method are also quite different from the procedures of typical thawing method for ordinary cell lines. In this course, we will offer a lecture on how to cryopreserve human iPS cells with a vitrification method and how to thaw them in 1-day course.

Host Division: Cell Engineering Division

Dates Number of trainees
From September 6, 2019  (One day) 1 people
Training course for basic technologies required for Arabidopsis research
Optional course: Training course for cultivation of Brachypodium distachyon Bd21

This is a training course for the researchers who are willing to study the plant science using Arabidopsis.
<Optional course (at your request)>
Trainees will learn the cultivation procedure for Brachypodium distachyon, a model of monocot plants.

Host Division: Experimental Plant Division

Dates Number of trainees
From September 4 to 5 AM, 2019  (2 consecutive days) 1 people
<Optional course> From September 5 PM, 2019 1 people
Technical training course for maintenance of plant cell cultures and transformation of Arabidopsis T87 cells
Optional course: Technical training course for the cryopreservation of tobacco BY-2 cells

This course presents basic methods to maintain Arabidopsis T87 and Tobacco BY2 suspension cultured cells (day 1) and applied methods for transformation technology of T87 cell (day 2).
<Optional course (at your request)>
Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen.

Host Division: Experimental Plant Division

Dates Number of trainees
From September 2 to 3 AM, 2019  (3 consecutive days) 5 people
<Optional course> From September 3 PM to 4 AM, 2019 2 people