************************************************** ****************** RIKEN BioResource Research Center Mouse Mail News (e-mail version) No. 174 ************************************************** ****************** A fruitful fall. There was news of Dr. Akira Yoshino’s Nobel Prize in Chemistry. I am very happy and proud to have a Japanese Nobel Prize winner this year. The latest information from RIKEN BRC Laboratory Animal Development Office. ━━━━ Next Next ━━━━━━━━━━━━━━━━━━━━━━━━ ● Special feature Mouse of the month   “Mice expressing 3xFLAG-dCas9 useful for analyzing molecular interactions on specific genomic regions” ● Today’s tool for functional analysis ● October 28 (Monday) Announcement of establishment anniversary ● Participation report: INFRAFRONTIER satellite workshop at the IMGC 2019 ● User achievements ● Newly deposited mouse strain ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 特集 Special Feature Mouse of the Month (October 2019) ┗━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 3xFLAG-dCas9 expressing mice useful for analyzing molecular interactions on specific genomic regions RBRC10188 C57BL / 6-Gt (ROSA) 26Sor RBRC10189 C57BL / 6-Gt (ROSA) 26Sor RBRC10190 C57BL / 6-Gt (ROSA) 26Sor Depositary: Prof. Hotaka Fujii (Graduate School of Medicine, Hirosaki University) Click here for details  https://mus.brc.riken.jp/en/mouse_of_month/oct_2019_mm This month’s mouse back number  https://mus.brc.riken.jp/en/mouse_of_month If you would like to post on the mouse this month, Laboratory Animal Development Office (animal@brc.riken.jp) Please contact us. ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ ┃ Today’s tool for functional analysis ┗━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Intracellular oxidative stress imaging mouse RBRC05704 C57BL / 6J-Tg (3xARE / TK-NRF2 / luc) M2Tiw RBRC06161 C57BL / 6J-Tg (3xARE / TK-NRF2 / venus) F4Tiw Mice that can conditionally control increased ROS production RBRC09859 C57BL / 6J-Tg (CMV-cat, -SDHA <*> / Y215F) 1Hom Sperm-egg membrane fusion imaging mouse RBRC05763 STOCK Izumo1 Tg (Clgn-Izumo1 / mCherry) Osb Tg (CAG / Acr-EGFP) C3-N01-FJ002Osb Sperm acrosome-GFP: sperm mitochondria-DsRed double reporter mouse RBRC03743 B6D2-Tg (CAG / Su9-DsRed2, Acr3-EGFP) RBGS002Osb RBRC05861 B6.Cg-Tg (CAG / Su9-DsRed2, Acr3-EGFP) RBGS002Osb Today’s tool for functional analysis  https://mus.brc.riken.jp/en/todays_tool_and_model ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 28 October 28 (Monday) Announcement of establishment anniversary ┗━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ October 28 (Monday) will be closed for the anniversary of establishment. We apologize for the inconvenience and thank you for your understanding. https://en.brc.riken.jp/news/notice_20191015_01.html ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 報告 Participation report: INFRAFRONTIER satellite workshop at the IMGC 2019 ┗━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Participation report: INFRAFRONTIER satellite workshop at the IMGC 2019  https://en.brc.riken.jp/news/report_20191008_01.html ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 成果 User achievements ┗━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Research results using mice provided by RIKEN BRC have been announced one after another. RIKEN BRC wants to help you discover new things by introducing your achievements am thinking. If you have published a paper or obtained a patent, please contact us. Doing. RBRC00445 C57BL / 6-Tg (CAG / Acr-EGFP) C3-N01-FJ002Osb Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prize KM. High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation. Sci Rep .; 9 (1): 12618, doi: 10.1038 / s41598-019-48772-3, 2019. https://doi.org/10.1038/s41598-019-48772-3 RBRC00753 B6; 129P2-Sema4d / Rbrc Zhang C, Qiao H, Guo W, Liu Y, Yang L, Liu Y, Jin B, Fu M, Wang G, Li W. CD100-plexin-B1 induces epithelial-mesenchymal transition of head and neck squamous cell carcinoma and promotes metastasis. Cancer Lett .; 455: 1-13, doi: 10.1016 / j.canlet.2019.04.013, 2019. https://doi.org/10.1016/j.canlet.2019.04.013 RBRC01834 C57BL / 6-Tg (CAG-flpe) 36Ito / ItoRbrc Sakata D, Uruno T, Matsubara K, Andoh T, Yamamura K, Magoshi Y, Kunimura K, Kamikaseda Y, Furue M, Fukui Y. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol .; 144 (4): 1130-1133.e8, doi: 10.1016 / j.jaci. 2019.06.031, 2019. https://doi.org/10.1016/j.jaci.2019.06.031 RBRC05555 B6; B6129-Ezh2 / Hko [B6-Ezh2 floxed] Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y. Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-wild-type Diffuse Astrocytic Gliomas. Cancer Res .; 79 (19): 4814-4827, doi: 10.1158 / 0008-5472. CAN-19-1272, 2019. https://doi.org/10.1158/0008-5472.CAN-19-1272 RBRC05663 B6.Cg-Bcl6 Otsuka K, Yamada A, Saito M, Ushio A, Sato M, Kisoda S, Shao W, Tsunematsu T, Kudo Y, Arakaki R, Ishimaru N. Ascl2-Regulated Follicular Helper T Cells Promote Autoimmunity in a Murine Model for Sjögren’s Syndrome. Am J Pathol., Doi: 10.1016 / j.ajpath.2019.08.008, 2019. https://doi.org/10.1016/j.ajpath.2019.08.008 RBRC05704 C57BL / 6J-Tg (3xARE / TK-NRF2 / luc) M2Tiw Suzuki T, Muramatsu A, Saito R, Iso T, Shibata T, Kuwata K, Kawaguchi SI, Iwawaki T, Adachi S, Suda H, Morita M, Uchida K, Baird L, Yamamoto M. Molecular Mechanism of Cellular Oxidative Stress Sensing by Keap 1. Cell Rep .; 28 (3): 746-758.e4, doi: 10.1016 / j.celrep. 2019.06.047, 2019. https://doi.org/10.1016/j.celrep.2019.06.047 RBRC06344 C57BL / 6-App Hamaguchi T, Tsutsui-Kimura I, Mimura M, Saito T, Saido TC, Tanaka KF. AppNL-G-F / NL-G-F mice overall do not show impaired motivation, but cored amyloid plaques in the striatu