{"id":4855,"date":"2019-10-11T17:45:28","date_gmt":"2019-10-11T08:45:28","guid":{"rendered":"http:\/\/penqe.com\/riken\/brc_wp3\/?p=4855"},"modified":"2019-10-11T17:45:28","modified_gmt":"2019-10-11T08:45:28","slug":"%e5%8a%b9%e7%8e%87%e7%9a%84%e3%81%aapcr%e3%82%af%e3%83%ad%e3%83%bc%e3%83%8b%e3%83%b3%e3%82%b0%e3%81%ae%e3%81%9f%e3%82%81%e3%81%ae%e6%96%b0%e8%a6%8f%e9%96%8b%e7%99%ba%e3%83%99%e3%82%af%e3%82%bf","status":"publish","type":"post","link":"http:\/\/web.brc.riken.jp\/en\/archives\/4855","title":{"rendered":"\u52b9\u7387\u7684\u306aPCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u305f\u3081\u306e\u65b0\u898f\u958b\u767a\u30d9\u30af\u30bf\u30fc"},"content":{"rendered":"<ul>\n \t<li>PCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u65b0\u898f\u30d9\u30af\u30bf\u30fc\u304c\u3001\u4eac\u90fd\u7523\u696d\u5927\u5b66\u751f\u547d\u79d1\u5b66\u90e8\u306e\u672c\u6a4b \u5065 \u5148\u751f\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u3001\u672c\u5e744\u6708\u306b\u8ad6\u6587\u3068\u3057\u3066\u767a\u8868\u3055\u308c\u307e\u3057\u305f(\u4e0b\u8a18\u6587\u732e\u7b49\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044)\u3002TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u4e26\u3073\u306b\u517c\u7528\u306e3\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<a href=\"https:\/\/dna.brc.riken.jp\/ja\/#\">[more\u2026]<\/a><\/li>\n<\/ul>\n<div id=\"EXR0101j\">\n<ul>\n \t<li style=\"list-style-type: none;\">\n<ul>\n \t<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\n<ul>\n \t<li>TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/dna.brc.riken.jp\/DataSheet\/RDB17479\" data-wpel-link=\"internal\">pCRT (cat# RDB17479)<\/a>\n\u5236\u9650\u9175\u7d20Xcm I\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n \t<li>\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/dna.brc.riken.jp\/DataSheet\/RDB17481\" data-wpel-link=\"internal\">pCRZero (cat# RDB17481)<\/a>\n\u5236\u9650\u9175\u7d20EcoR V\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n \t<li>TA-\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u517c\u7528\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/dna.brc.riken.jp\/DataSheet\/RDB17480\" data-wpel-link=\"internal\">pCRZeroT (cat# RDB17480)<\/a>\n\u5236\u9650\u9175\u7d20Xcm I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u3001\u5236\u9650\u9175\u7d20Sma I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u305d\u308c\u305e\u308c\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/exr0101map.png\" alt=\"\" width=\"100%\" \/>\n<ul>\n \t<li>\u8ad6\u6587\nMotohashi, K. A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417, 2019. PMID: 31015513.<\/li>\n \t<li>\u53c2\u8003web\u30b5\u30a4\u30c8\n<a class=\"ext-link wpel-icon-right\" href=\"https:\/\/www.kyoto-su.ac.jp\/news\/20190424_400a_news.html\" target=\"_new\" rel=\"follow noopener noreferrer\" data-wpel-link=\"external\">\u751f\u547d\u79d1\u5b66\u90e8 \u672c\u6a4b \u5065 \u6559\u6388\u304cPCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u305f\u3081\u306e\u65b0\u3057\u3044\u30d9\u30af\u30bf\u30fc\u3092\u958b\u767a\u3057\u307e\u3057\u305f (\u4eac\u90fd\u7523\u696d\u5927\u5b662019\/4\/24)<\/a><\/li>\n<\/ul>\n<\/div>","protected":false},"excerpt":{"rendered":"PCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u65b0\u898f\u30d9\u30af\u30bf\u30fc\u304c\u3001\u4eac\u90fd\u7523\u696d\u5927\u5b66\u751f\u547d\u79d1\u5b66\u90e8\u306e\u672c\u6a4b \u5065 \u5148\u751f\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u3001\u672c\u5e744\u6708\u306b\u8ad6\u6587\u3068\u3057\u3066\u767a\u8868\u3055\u308c\u307e\u3057\u305f(\u4e0b\u8a18\u6587\u732e\u7b49\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044)\u3002TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u4e26\u3073\u306b\u517c\u7528\u306e3\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002[more\u2026] \u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9 TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; pCRT (cat# RDB17479) \u5236\u9650\u9175\u7d20Xcm I\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; pCRZero (cat# RDB17481) \u5236\u9650\u9175\u7d20EcoR V\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 TA-\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u517c\u7528\u30d9\u30af\u30bf\u30fc; pCRZeroT (cat# RDB17480) \u5236\u9650\u9175\u7d20Xcm I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u3001\u5236\u9650\u9175\u7d20Sma I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u305d\u308c\u305e\u308c\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u8ad6\u6587 Motohashi, K. A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417, 2019. PMID: 31015513. \u53c2\u8003web\u30b5\u30a4\u30c8 \u751f\u547d\u79d1\u5b66\u90e8 \u672c\u6a4b \u5065 \u6559\u6388\u304cPCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u305f\u3081\u306e\u65b0\u3057\u3044\u30d9\u30af\u30bf\u30fc\u3092\u958b\u767a\u3057\u307e\u3057\u305f (\u4eac\u90fd\u7523\u696d\u5927\u5b662019\/4\/24)","protected":false},"author":12,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"categories":[6],"tags":[],"class_list":["post-4855","post","type-post","status-publish","format-standard","hentry","category-dna-announce","entry"],"acf":[],"_links":{"self":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts\/4855","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=4855"}],"version-history":[{"count":0,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts\/4855\/revisions"}],"wp:attachment":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=4855"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/categories?post=4855"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/tags?post=4855"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}