{"id":4435,"date":"2019-10-21T18:40:44","date_gmt":"2019-10-21T09:40:44","guid":{"rendered":"http:\/\/penqe.com\/riken\/brc_wp3\/?page_id=4435"},"modified":"2023-06-14T15:32:48","modified_gmt":"2023-06-14T06:32:48","slug":"anthology","status":"publish","type":"page","link":"http:\/\/web.brc.riken.jp\/en\/method\/anthology","title":{"rendered":"Protocols and Manuals"},"content":{"rendered":"
\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n
Experimental Animal (Mouse)<\/th>\r\n <\/tr>\r\n
<\/span>Genetic Background<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Genotyping PCR<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Frozen Sperm Data<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Frozen Embryo Data<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Breeding characters<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Electrophoretic testing for biochemical markers<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>FACS Analysis<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Microbiological monitoring<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Microinjection of mRNA into fertilized or cloned zygotes<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Collection of mouse spermatogenic cells<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Embryo cryopreservation(vitrification)\/thawing<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Somatic cell nuclear transfer<\/a><\/td>\r\n <\/tr>\r\n
Experimental Plant<\/th>\r\n <\/tr>\r\n
<\/span>RIKEN BRC plant cell line documentation<\/a><\/td>\r\n <\/tr>\r\n
<\/span>A Set of Natural Arabidopsis Accessions<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Growth Method for Brachypodium<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Maps for RAFL clones<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Sequence of pPCVICEn4HPT (RTF)<\/a><\/td>\r\n <\/tr>\r\n
Cell Line<\/th>\r\n <\/tr>\r\n
(under construction)<\/td>\r\n <\/tr>\r\n
Genetic Material<\/th>\r\n <\/tr>\r\n
<\/span>BAC Related Information<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Genomic DNA Preparation<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Recombinant Virus Protocols<\/a><\/td>\r\n <\/tr>\r\n
<\/span>Purification of Recombinant Tubulin by Dr. Etsuko Muto<\/span><\/a><\/td>\r\n <\/tr>\r\n
<\/span>Sequencing & PCR primers<\/a><\/td>\r\n <\/tr>\r\n
<\/span>List of references of genetic materials<\/a><\/td>\r\n <\/tr>\r\n
Microbe<\/th>\r\n <\/tr>\r\n
<\/span>HOW TO REVIVE LYOPHILIZED OR L-DRIED CULTURES<\/a><\/td>\r\n <\/tr>\r\n
<\/span>HOW TO REVIVE FROZEN-THAWED CULTURES<\/a><\/td>\r\n <\/tr>\r\n <\/tbody>\r\n <\/table>\r\n<\/section>\r\n","protected":false},"excerpt":{"rendered":"Experimental Animal (Mouse) Genetic Background Genotyping PCR Frozen Sperm Data Frozen Embryo Data Breeding characters Electrophoretic testing for biochemical markers FACS Analysis Microbiological monitoring Microinjection of mRNA into fertilized or cloned zygotes Collection of mouse spermatogenic cells Embryo cryopreservation(vitrification)\/thawing Somatic cell nuclear transfer Experimental Plant RIKEN BRC plant cell line documentation A Set of Natural Arabidopsis Accessions Growth Method for Brachypodium Maps for RAFL clones Sequence of pPCVICEn4HPT (RTF) Cell Line (under construction) Genetic Material BAC Related Information Genomic DNA Preparation Recombinant Virus Protocols Purification of Recombinant Tubulin by Dr. Etsuko Muto Sequencing & PCR primers List of references of genetic materials Microbe HOW TO REVIVE LYOPHILIZED OR L-DRIED CULTURES …

Continue reading “Protocols and Manuals”<\/span><\/a><\/p>","protected":false},"author":12,"featured_media":0,"parent":5067,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4435","page","type-page","status-publish","hentry","entry"],"acf":[],"_links":{"self":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=4435"}],"version-history":[{"count":22,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4435\/revisions"}],"predecessor-version":[{"id":9731,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4435\/revisions\/9731"}],"up":[{"embeddable":true,"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/5067"}],"wp:attachment":[{"href":"http:\/\/web.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=4435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}